The PM1700 ExcelBand™ All Blue Broad Range Protein Marker is a blue protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 240 kDa in Tris-Glycine buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer).
The PM1700 ExcelBand™ All Blue Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
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Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
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Two enhanced bands — 72 kDa and 25 kDa
Contents
Approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM1700 ExcelBand™ All Blue Broad Range Protein Marker resolves 12 major bands in SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months
-20°C for long term storage
Specification
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Cat. No.
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PM1700
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Series Name
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ExcelBand™
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Product Size
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2 x 250 μl
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MW Range
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10 – 240 kDa
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Band Number
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12
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Band Color
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All blue
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Enhanced Bands
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25, 72 kDa
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