The FluoroStain™ DNA Fluorescent Staining Dye is designed to be a safer replacement for conventional Ethidium bromide (EtBr) which poses a significant health and safety hazard for its users. The FluoroStain™ DNA Fluorescent Staining Dye offers at least 10 times sensitivity in DNA detection levels, and is capable of detecting double stranded DNA (dsDNA) fragments up to 0.04 ng in electrophoresis analysis. The FluoroStain™ DNA Fluorescent Staining Dye shows a high specificity to the dsDNA, with negligible background signal, making the destaining process entirely optional. FluoroStain™ DNA Fluorescent Staining Dye is compatible with both the conventional ultra violet gel-illuminating systems as well as the less harmful long wave length blue light illumination systems. The emission when bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.

Features

• Excellent for post staining

• Sensitivity: 0.04 ng DNA

• A safer alternative to EtBr

• Compatibility: suitable to blue or UV light

• Increased cloning efficiency (blue light)

Storage

Protected from light
4°C for 12 months
-20°C for 24 months

Sensitivity of FluoroStain™

The FluoroStain™ DNA Fluorescent Staining Dye (DS1000) shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment. 

Excitation and emission spectrum of FluoroStain™ 

FluoroStain™ DNA Fluorescent Staining Dye (DS1000) emission as bound to dsDNA is 522 nm while its excitation peaks are at 270, 370 and 497 nm. 

Non-mutagenicity of FluoroStain™

FluoroStain™ DNA Fluorescent Staining Dye (DS1000) is proofed for their safety (non-mutagenicity) using Ames test. However, it must be noted that since solvent may penetrate the skin, it is recommended that users wear gloves when using the fluorescent dyes.

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