FluoroVue™ Nucleic Acid Gel Stain (10,000X) is specially designed for in-gel use and is a safer replacement for conventional Ethidium bromide (EtBr), which poses a significant health and safety hazard to its users. It is a fluorescent stain which offers highly sensitive detection of double-stranded or single-stranded DNA and RNA in a convenient manner. FluoroVue™ Nucleic Acid Gel Stain offers high sensitivity that is several times greater than EtBr. 

FluoroVue™ Nucleic Acid Gel Stain is compatible with both conventional UV gel-illumination systems as well as harmless long wavelength blue light illumination systems, like B-BOX™. When bound to nucleic acids, FluoroVue™ Nucleic Acid Gel Stain has a fluorescent excitation maximum of 250 and 482 nm, and an emission maximum of 509 nm. Therefore, it can replace EtBr without the need of changing existing lab imaging systems.

Features

• Excellent for in-gel staining

• Sensitivity: 0.14 ng (DNA) or 1 ng (total RNA)

• A safer alternative to EtBr

• Compatibility: suitable to blue or UV light

• Increased cloning efficiency (blue light)

Storage

Protected from light
4°C for 12 months
-20°C for 24 months

Sensitivity of FluoroVue™

The FluoroVue™ Nucleic Acid Gel Stain (NS1000) shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment. 

Excitation and emission spectrum of FluoroVue™ 

FluoroVue™ Nucleic Acid Gel Stain (NS1000) has a fluorescent excitation maxima of ~250 and ~482 nm, and an emission maximum of ~509 nm. Therefore, it can replace EtBr without the need for changing existing lab imaging systems. 

Non-mutagenicity of FluoroVue™

FluoroVue™ Nucleic Acid Gel Stain (NS1000) is proofed for their safety (non-mutagenicity) using Ames test. However, it must be noted that since solvent may penetrate the skin, it is recommended that users wear gloves when using the fluorescent dyes.

 Contents

Use collapsible tabs for more detailed information that will help customers make a purchasing decision.

Ex: Shipping and return policies, size guides, and other common questions.