FluoroDye™ DNA Fluorescent Loading Dye is a ready-to-use 6X DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye™ Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye™ DNA Fluorescent Loading Dye allows the user to immediately visualize electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye™ DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye™ emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.

Features

• Excellent for premix with DNA samples

• Sensitivity: 0.14 ng (DNA)

• A safer alternative to EtBr

• Compatibility: suitable to blue or UV light

• Increased cloning efficiency (blue light)

Composition 

FluoroDye™ DNA Fluorescent Loading Dye is stored in 6X concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing Bromophenol blue, Xylene cyanol FF and Orange G as tracking dyes.

Storage

Protected from light
-20°C for 24 months

Sensitivity of FluoroDye™

FluoroDye™ DNA Fluorescent Loading Dye (DL5000) shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment. 

Excitation and emission spectrum of FluoroDye™ 

FluoroDye™ DNA Fluorescent Loading Dye (DL5000) emission as bound to dsDNA is 522 nm while its excitation peaks are at 270, 370 and 497 nm. 

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